User:Le Pensive3

https://drive.google.com/open?id=1hkf8PZLnAEbhn9kv9b6oXe_oZnHKJlGsua_xSiME5qA

Peer Review (Tianxiao Yang):

Main problem with language--

There are some instances of opinionated language. For instance "Another problem with the technique is that light stimulation produces a synchronous activation of infected cells and this removes any individual cell properties of activation among the population affected. Therefore, it is difficult to understand how the cells in the population affected communicate with one another or how their phasic properties of activation may relate to the circuitry being observed." here I would recommend being more neutral in your approach. Perhaps instead of acknowledging something is difficult or a problem, talk about what exactly is difficult or a problem. Less statements and more facts.

"While this extension of optogenetics remains rather novel, there are various conceptual methodologies that may be robust. "

Again, who's to really say just how novel or robust this is? I believe these words are a tad bit too connotatively charged. Dial it down a bit. Perhaps use "new" and "more useful".

Overall, this is a really solid contribution to the article. Just pay attention to how you use your words. You are doing really well. Keep it up.

Peer Review suggestions on Optogenetics (Juka Kim):

After Line 161:

- "...due to its high ceiling..": I think because this is a Wikipedia article, "high ceiling" is a little bit too fancy here

- "With that being said": seems a little too informal, so it may be a good idea to substitute that.

- "Integration of opsin into subcellular compartments such as dendrites, somata or axon terminals will provide a more robust understanding of neuronal circuitry": maybe include links here to other Wikipedia articles

After Line 270:

- "The optogenetic toolkit has proven pivotal for the field of neuroscience as it precise manipulation of neuronal excitability however this technique has been shown to extend outside neurons to an increasing number of proteins and cellular functions": I think this is a run-on sentence

I really enjoyed reading it, but you might want to try to explain things in slightly more accessible terms and sound less like a scientific article (More so when you talk about limitations). I also think you could add a lot more links to other Wikipedia definitions. Overall, I think it is very well written :)

Optogenetics Outline

1. Expansion of ‘Issues Section’ with a focus on possibilities or room for growth? (Concepts for a, b, c, d adapted from https://www.sfn.org/~/media/SfN/Documents/Short%20Courses/2013%20Short%20Course%20I/SC1%20Deisseroth.ashx)
   1. Specificity Issue
      1. Subcellular targeting guidance strategies to allow for selective expression in subcellular compartments (dendrites, somata, axon terminals) is difficult to do without confounding expression in axons
   2. Connectomic Elucidation
      1. Optogenetics has the capability of revealing brainwide wiring networks/projections derived from the stimulation/optogenetic control of some population. fMRI is currently an option however this readout has spatial and temporal resolution issues. The ability to associate a certain cell’s behavior with the rest of its network and possibly “close the loop” to engineer a feedback system spurred by optogenetic control can have far reaching ramifications.
   3. Expanding optic spectrum
      1. Incorporation of infrared light allows for deeper penetration, increased resolution via scattering reduction. This has proven successful for optogenetics without the use of opsin however expansion into this domain will increase the versatility of this technique.
   4. Biochemical signaling
      1. Understanding and subsequently influencing/adapting a biochemical pathway via G-protein coupled reecptors, intracellular signaling etc. can open up a completely new field of use for the technique
2. 2.      Illuminating Cell Signaling Pathways (http://www.nature.com/nrm/journal/v15/n8/full/nrm3837.html)
   1. Neuronal optogenetics tool can only influence membrane potentials however the technique has the capability to elucidate processes in cellular and developmental biology
      1. Protein Localization
      2. Post-translational modification
      3. GTP Loading
   2. Different Strategies for Optogenetic Inputs (Fig. 1)
      1. Inducible Protein Association
      2. Gene Expression
      3. Clustering-based activation
      4. Sequestration-based inhibition
      5. Conformation Change+
   3. Optogenetic Signal Control over time (Fig. 4)
      1. Cellular decision changes (proliferation vs. differentiation)
      2. Downstream signaling up/down regulation
      3. “Closing the loop”

Week 4's Homework Assignment:

LIVE EDITTED the Carbapenem page.

In addition, here is the link to the Talk:Carbapenem page where I've spoken a little to my justification for making the edits.

Initial Bibliography with 5 sources for Optogenetics article:

1. Illuminating cell signaling with optogenetic tools
2. The promise of optogenetics in cell biology: interrogating molecular circuits in space and time
3. Spotlighted Brains: Optogenetic Activation and Silencing of Neurons
4. Optogenetics Summary
5. Optogenetics : light-sensing proteins and their applications (will be using the google preview specifically Chapter 8 "General Description: Future Prospects of Optogenetics" and Chapters 16 - 22 "Optogenetics in Neuroscience"

-------------

Hello Dr. Tienson!

Week 2's Homework Assignment:

Plausible articles to edit include:

1. Neurotransmitter - The page is evaluated as 'top-importance' across disciplines including cell signaling, neuroscience, molecular and cell biology, psychiatry and pharamacology but fails to delve into the biochemical intricacies that are involved. I did a very brief google search and found two of these resources (Biochemistry, Neurotransmitters and Neuromodulators and the Biochemistry of Neurotransmitter Secretion) that may prove useful in augmenting the page. Would these resources be appropriate?
2. Antimicrobial_Resistance - This page is evaluated as 'top/high - importance' in the fields of microbiology, molecular and cell biology and medicine. Although there may not be a lot of room to expand here, I found this (rather old) paper titled The origins and molecular basis of antibiotic resistance and another shorter piece titled The biochemical basis of antimicrobial and bacterial resistance that may provide a fair amount of information to expand the topic through a lens of biochemistry.
3. Optogenetics - As a Neuroscience major, the recent advent of optogenetics has revolutionized the way we are able to attack and subsequently understand circuitry. Although we don't utilize the technique in my lab, it's one that I've become intimately familiar with as 50% of the papers presented in our journal club employ the technique in some way, shape or form. From what I understand, the technique also has a high ceiling in the field of Biochemistry and that would be my focus if I were to edit this article. The following are possible resources for this venture:
   • Illuminating cell signaling with optogenetic tools
   • The promise of optogenetics in cell biology: interrogating molecular circuits in space and time
   • Spotlighted Brains: Optogenetic Activation and Silencing of Neurons