Talk:Epitope mapping

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Another method[edit]

Mapping Epitopes with H/D-Ex Mass Spec. May 2011 describes using deuteration followed by mass-spec to map at medium resolution (10-15 residues). "Some limitations to H/D-Ex include heavily glycosylated proteins in which it may be difficult to identify glycosylated peptides after digestion, and heavily di-sulfide-bonded proteins, which are difficult to digest and make it difficult to identify peptic fragments. Also, proteins larger than 100 kDa may have reduced sequence coverage." - Rod57 (talk) 06:13, 17 May 2011 (UTC)[reply]